核苷酸色譜分析

Oligonucleotide Ladder Standard

Column:ACE Excel 3 Oligo

Dimensions:150 x 4.6 mm

Mobile Phases:

A: 80 mM triethylammonium acetate in H20 {pH 7.0)

B: 80 mM triethylammonium acetate in MeCN

Gradient: 

Time（mins）	%B
0	12
30	15
36	15
36.5	12
66.5	12

System Dwell Volume:550μL

Flow Rate:1.0 ml/min

Injection:20μL

Temperature:60°C

Detection:UV, 260 nm

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